Medium Performance
ISO/TS 22964 STANDARD: E.sakazakii agar is a chromogenic medium for detection of E.sakazakii in food, mainly powdered milk, according to the ISO/TS 22964 standard.
INTENSE BLUE COLONIES: E.sakazakii will grow in blue colonies while most other bacteria will be inhibited or grow in colourless colonies, after Incubation at 44°C for 24h.
Medium Description
CHROMagar is a trademark of Dr A. Rambach
Last Update : 23 - Jan - 2012
Focus on E.sakazakii
« Enterobacter sakazakii is a gram-negative, non-spore-forming bacterium belonging to the
Enterobacteriaceae family <……> It has been implicated in outbreaks causing meningitis or
enteritis, especially in infants. In the few outbreaks reported 20% to >50% of the infants who
contracted the disease died. For survivors, severe lasting complications can result including
neurological disorders. The outcome related to adult disease seems to be significantly milder.”
« The infant food industry should be encouraged to reduce the concentration and prevalence of
E. sakazakii in both the manufacturing environment and powdered infant formula. To this end,
the infant food industry should consider implementing an effective environmental monitoring
programme and the use of Enterobacteriaceae rather than coliform testing as an indicator of
hygienic control in factory production lines. »Extracts From WHO MicrobiaL Risk Assesment Feb-2007
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